There have always been theories that Asuka & Shinji had sex, but usually they revolve around the mentions of Asuka's period and her mentions.
In the movie they were hinting at that Shinji and Asuka had sex offscreen in the tv series. Did this realy happen? what do you guys think?.
I can't say for certain, but around the scene where Asuka, Rei, and Misato all appear in sequence in front of Shinji saying "don't you want to become one with me.
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I'm too lazy to edit the video, but here's something else worthy of note. In the biblical fable of the wall of Jericho, it's brought down after seven.